Jourdil N, Pinteur B, Vincent F, Marka C, Bessard G
Laboratoire de Pharmacologie, Centre Hospitalier Universitaire de Grenoble, France.
J Chromatogr. 1993 Mar 5;613(1):59-65. doi: 10.1016/0378-4347(93)80197-c.
A simple procedure is described that permits the simultaneous determination of trimipramine and its two major metabolites, desmethyl- and hydroxytrimipramine, in human plasma or red blood cells (RBCs) at therapeutic concentrations. The extracted biological fluids are injected into a capillary gas chromatograph with an OV-1 fused-silica column coupled to a nitrogen-phosphorus-selective detector. The limit of determination for trimipramine is 3 ng/ml and for that desmethyl- and hydroxytrimipramine is 4 ng/ml. The method permits the RBC/plasma ratios to be determined and to be correlated with the clinical response.
本文描述了一种简单的方法，可同时测定人血浆或红细胞（RBC）中治疗浓度的三甲丙咪嗪及其两种主要代谢产物去甲基三甲丙咪嗪和羟基三甲丙咪嗪。将提取的生物样品注入配有OV-1熔融石英柱并与氮磷选择性检测器相连的毛细管气相色谱仪中。三甲丙咪嗪的检测限为3 ng/ml，去甲基三甲丙咪嗪和羟基三甲丙咪嗪的检测限为4 ng/ml。该方法可测定红细胞/血浆比值，并将其与临床反应相关联。
