Scheide J I
Department of Biology, Central Michigan University, Mt. Pleasant 48859.
J Exp Zool. 1993 Jan 1;265(1):88-91. doi: 10.1002/jez.1402650113.
The inner opercular epithelium of the killifish, Fundulus heteroclitus, may be separated as a single epithelial sheet and mounted in an Ussing-style chamber with the short-circuit current (Isc) approximating chloride secretion. Steady state opercular Isc values in seawater-adapted killifish were higher in tissues removed from fish in the afternoon than those observed in the morning. Opercular conductance was also higher in the afternoon than the morning. The opercular steady state Isc from killifish on a 13:11 light:dark cycle exhibited a diel variation with the peak in the middle of the light cycle and a nadir near the end of the dark phase. Temperature and salinity remained constant during the experimental period. Daily variation cued by the photoperiod should be considered when using this preparation as an experimental model for ion transport in the gill of a seawater-adapted fish.
将底鳉（Fundulus heteroclitus）的鳃盖内上皮作为单层上皮分离出来，并安装在乌斯森（Ussing）式小室中，短路电流（Isc）接近氯化物分泌。下午从适应海水的底鳉身上取出的组织中，鳃盖的稳态Isc值高于上午观察到的值。下午的鳃盖电导率也高于上午。在13:11光照:黑暗周期下的底鳉的鳃盖稳态Isc呈现出昼夜变化，在光照周期的中间达到峰值，在黑暗阶段接近结束时达到最低点。在实验期间，温度和盐度保持恒定。当使用该制剂作为适应海水的鱼类鳃中离子转运的实验模型时，应考虑由光周期引起的每日变化。
