Allred D R
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, 32611-0880, USA.
Methods. 1997 Oct;13(2):177-89. doi: 10.1006/meth.1997.0510.
Intraerythrocytic parasites, such as Babesia bovis, modify the erythrocyte plasma membrane structurally, antigenically, and functionally. For such parasites the infected erythrocyte surface also is thought to be a primary site for interaction with the host immune system. These properties demand characterization of the various alterations to understand the overall host-parasite interaction, immunity to disease or infection, and bases for parasite persistence. A paucity of adequate methods exists for characterization of parasite-derived components of the parasitized erythrocyte surface. To facilitate such studies we developed or modified several techniques to detect, identify, and localize parasite-induced alterations on the B. bovis-infected erythrocyte surface. These methods, which we present here, should be adaptable to a variety of intraerythrocytic parasite-host combinations.
红细胞内寄生虫，如牛巴贝斯虫，会在结构、抗原性和功能上改变红细胞质膜。对于这类寄生虫，被感染红细胞的表面也被认为是与宿主免疫系统相互作用的主要部位。这些特性需要对各种改变进行表征，以了解宿主与寄生虫的整体相互作用、对疾病或感染的免疫力以及寄生虫持续存在的基础。目前缺乏足够的方法来表征被寄生红细胞表面的寄生虫衍生成分。为了便于此类研究，我们开发或改进了几种技术，以检测、识别和定位牛巴贝斯虫感染红细胞表面的寄生虫诱导改变。我们在此介绍的这些方法应该适用于各种红细胞内寄生虫与宿主的组合。
