Herrera-Estrella A, Goldman G H, van Montagu M, Geremia R A
Laboratorium voor Genetica, Universiteit Gent, Belgium.
Mol Microbiol. 1993 Feb;7(4):515-21. doi: 10.1111/j.1365-2958.1993.tb01142.x.
A molecular karyotype for three different Trichoderma species (T. harzianum, T. viride, and T. reesei) was determined by using two different systems: countour-clamped electric-field and rotating-electrode electrophoresis. Six chromosomal DNA bands were observed in T. harzianum and T. reesei and five in T. viride. The sizes of these molecules were estimated by their mobility relative to the Schizosaccharomyces pombe chromosomes and ranged between 2.2 and 7.4 megabase pairs (mbp). The estimated genome sizes range from 31 to 39 mbp. A number of genes were located in the different chromosomes by means of Southern analysis. The implications of these findings are discussed.
利用两种不同系统（轮廓钳制电场电泳和旋转电极电泳）测定了三种不同木霉属物种（哈茨木霉、绿色木霉和里氏木霉）的分子核型。在哈茨木霉和里氏木霉中观察到六条染色体DNA条带，在绿色木霉中观察到五条。通过它们相对于粟酒裂殖酵母染色体的迁移率估计了这些分子的大小，范围在2.2至7.4兆碱基对（mbp）之间。估计的基因组大小在31至39兆碱基对之间。通过Southern分析将许多基因定位到不同的染色体上。讨论了这些发现的意义。
