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里氏木霉电泳核型的染色体及遗传分析:纤维素酶和木聚糖酶基因的定位

Chromosomal and genetic analysis of the electrophoretic karyotype of Trichoderma reesei: mapping of the cellulase and xylanase genes.

作者信息

Carter G L, Allison D, Rey M W, Dunn-Coleman N S

机构信息

Genencor International, South San Francisco, California 94080.

出版信息

Mol Microbiol. 1992 Aug;6(15):2167-74. doi: 10.1111/j.1365-2958.1992.tb01390.x.

Abstract

An electrophoretic karyotype has been established for Trichoderma reesei strain QM6a, and several of its derivatives, by pulsed-field gel electrophoresis. All strains examined appear to have seven chromosomes with a total genome size of approximately 33 megabases (Mb). The sizes of the chromosomal bands in strain QM6a are approximately 6.2, 6.0, 5.1, 4.2 (doublet), 3.6 and 3.2 Mb. Genes encoding the cellulase complex and xylanases of T. reesei have been mapped, as have several other genes.

摘要

通过脉冲场凝胶电泳,已为里氏木霉QM6a菌株及其几个衍生物建立了电泳核型。所有检测的菌株似乎都有七条染色体,基因组总大小约为33兆碱基(Mb)。QM6a菌株中染色体条带的大小约为6.2、6.0、5.1、4.2(双峰)、3.6和3.2 Mb。编码里氏木霉纤维素酶复合物和木聚糖酶的基因已被定位,其他几个基因也已被定位。

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