Becker R J, Cooper A J, Starzyk M J
Department of Biological Sciences, Northern Illinois University, DeKalb 60115-2861.
Microbios. 1993;73(295):123-33.
Thermus rubens has previously been reported to exhibit bacteriocin-like antagonism against other members of the genus Thermus when assayed via the deferred antagonism technique. Bacteriocin activity was not detectable in various age batch culture supernatants assayed by simple spotting procedures. Successful cell-free isolation was accomplished by way of a procedure employing French press disruption of T. rubens cells. Ultrafiltration and molecular exclusion chromatographic studies ascribed a high molecular weight to the agent which was found to be completely precipitable by ammonium sulphate. Activity was totally sedimentable upon ultracentrifugation. Transmission electron microscopic examination of column fractions containing high specific activity revealed numerous circular, apparently trilaminar, structures indicative of membrane vesicles.
先前有报道称，当通过延迟拮抗技术进行检测时，鲁氏栖热菌对栖热菌属的其他成员表现出类细菌素拮抗作用。通过简单的点样程序检测各种不同培养阶段的培养上清液时，未检测到细菌素活性。通过采用法国压榨机破碎鲁氏栖热菌细胞的程序成功实现了无细胞分离。超滤和分子排阻色谱研究表明，该因子具有高分子量，并且发现其可被硫酸铵完全沉淀。经超速离心后，活性完全沉降。对含有高比活性的柱级分进行透射电子显微镜检查，发现了许多圆形的、明显为三层的结构，表明存在膜泡。
