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亚麻短杆菌产生的细菌素Linocin M18的分离与特性研究

Isolation and characterization of Linocin M18, a bacteriocin produced by Brevibacterium linens.

作者信息

Valdés-Stauber N, Scherer S

机构信息

Institut für Mikrobiologie, München, Germany.

出版信息

Appl Environ Microbiol. 1994 Oct;60(10):3809-14. doi: 10.1128/aem.60.10.3809-3814.1994.

DOI:10.1128/aem.60.10.3809-3814.1994
PMID:7986050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC201890/
Abstract

Brevibacterium linens M18, isolated from red smear cheese, produces a substance that inhibits the growth of Listeria spp. and several coryneform and other gram-positive bacteria. No gram-negative bacteria were inhibited. The substance is heat labile, sensitive to proteolytic enzymes, and stable between pH 3 and 12. High levels of this bacteriocin, named Linocin M18, were obtained in the stationary growth phase. Linocin M18 was purified by ultrafiltration, ultracentrifugation, and gel filtration chromatography. In its native form, it is a proteinaceous aggregate with a high molecular weight. Fractions with Linocin M18 activity contained particles of 20 to 30 nm in diameter. The bacteriocin consists of a single protein subunit with a molecular mass of 31 kDa and an isoelectric point of 4.5 N-terminal sequence analysis yielded Met-Asn-Asn-Leu-Tyr-Arg-Glu-Leu-Ala-Pro-Ile-Pro-Gly-Pro-Ala-Ala-Ala-Glu- Ile. Significant homology with published sequences was lacking.

摘要

从红涂抹奶酪中分离得到的亚麻短杆菌M18能产生一种抑制李斯特菌属以及几种棒状杆菌和其他革兰氏阳性菌生长的物质。革兰氏阴性菌不受抑制。该物质对热不稳定，对蛋白水解酶敏感，在pH 3至12之间稳定。在稳定生长期可获得高产量的这种名为Linocin M18的细菌素。通过超滤、超速离心和凝胶过滤色谱法对Linocin M18进行了纯化。其天然形式是一种高分子量的蛋白质聚集体。具有Linocin M18活性的组分含有直径为20至30 nm的颗粒。该细菌素由一个分子量为31 kDa、等电点为4.5的单一蛋白质亚基组成。N端序列分析结果为Met-Asn-Asn-Leu-Tyr-Arg-Glu-Leu-Ala-Pro-Ile-Pro-Gly-Pro-Ala-Ala-Ala-Glu-Ile。与已发表序列缺乏显著同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf8/201890/a2916dad31ef/aem00027-0343-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf8/201890/25138610a960/aem00027-0342-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf8/201890/e9da234236ad/aem00027-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf8/201890/a2916dad31ef/aem00027-0343-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf8/201890/25138610a960/aem00027-0342-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf8/201890/e9da234236ad/aem00027-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf8/201890/a2916dad31ef/aem00027-0343-b.jpg

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