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通过ATP使葡萄糖调节蛋白Grp78与Grp78-IgE Fc复合物解离。

Dissociation of glucose-regulated protein Grp78 and Grp78-IgE Fc complexes by ATP.

作者信息

Toledo H, Carlino A, Vidal V, Redfield B, Nettleton M Y, Kochan J P, Brot N, Weissbach H

机构信息

Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.

出版信息

Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2505-8. doi: 10.1073/pnas.90.6.2505.

DOI:10.1073/pnas.90.6.2505
PMID:8460165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC46116/
Abstract

Recent studies have shown that ATP can dissociate dimers of the glucose-regulated protein Grp78 to monomers. In the present study, we have used purified recombinant Grp78 from Escherichia coli to investigate this reaction in more detail. During the course of the Grp78 dimer-monomer conversion, a stable Grp78 monomer-ATP complex is formed. Upon removal of the ATP, the Grp78 dimer is reformed. ADP, nonhydrolyzable ATP analogues, and GTP do not effect the dissociation of Grp78 dimers. A cell line that overproduces IgE Fc has been used to examine the nature of the Grp78-IgE Fc complexes present and the effect of ATP on them. Grp78-IgE Fc complexes ranging from 100 kDa to 300 kDa were observed by sucrose gradient analysis, suggesting that aggregate forms of Grp78 may be present in some of these complexes. Treatment of the extracts with ATP resulted in release of a Grp78 monomer from the complex. These results suggest that the dissociation of Grp78 oligomers by ATP may be involved in the function of Grp78 in protein translocation through the endoplasmic reticulum.

摘要

最近的研究表明，ATP可使葡萄糖调节蛋白Grp78的二聚体解离为单体。在本研究中，我们使用了从大肠杆菌中纯化得到的重组Grp78，以更详细地研究这一反应。在Grp78二聚体向单体的转化过程中，会形成一种稳定的Grp78单体-ATP复合物。去除ATP后，Grp78二聚体重新形成。ADP、不可水解的ATP类似物和GTP不会影响Grp78二聚体的解离。一种过量产生IgE Fc的细胞系已被用于研究存在的Grp78-IgE Fc复合物的性质以及ATP对它们的影响。通过蔗糖梯度分析观察到分子量在100 kDa至300 kDa之间的Grp78-IgE Fc复合物，这表明其中一些复合物中可能存在Grp78的聚集形式。用ATP处理提取物会导致复合物中释放出一个Grp78单体。这些结果表明，ATP介导的Grp78寡聚体解离可能参与了Grp78在内质网蛋白质转运中的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63c0/46116/51ebb858fe37/pnas01465-0419-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63c0/46116/084a815d261a/pnas01465-0418-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63c0/46116/87f2a0adb3a2/pnas01465-0418-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63c0/46116/51ebb858fe37/pnas01465-0419-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63c0/46116/084a815d261a/pnas01465-0418-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63c0/46116/87f2a0adb3a2/pnas01465-0418-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63c0/46116/51ebb858fe37/pnas01465-0419-a.jpg

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