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纯合突变嵌合小鼠的分析：免疫球蛋白重链连接区的缺失阻碍B细胞发育和抗体产生。

Analysis of homozygous mutant chimeric mice: deletion of the immunoglobulin heavy-chain joining region blocks B-cell development and antibody production.

作者信息

Jakobovits A, Vergara G J, Kennedy J L, Hales J F, McGuinness R P, Casentini-Borocz D E, Brenner D G, Otten G R

机构信息

Cell Genesys Inc., Foster City, CA 94404.

出版信息

Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2551-5. doi: 10.1073/pnas.90.6.2551.

DOI:10.1073/pnas.90.6.2551
PMID:8460171
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC46126/
Abstract

Using a recently described method for efficiently deriving homozygous targeted alleles in embryonic stem cells, we produced chimeric mice whose tissues were derived partially from embryonic stem cells bearing homozygous deletion of the mouse immunoglobulin heavy-chain joining (JH) region. Characterization of these chimeric mice indicated that homozygous JH deletion leads to arrest of B-cell development at an early stage, resulting in a total lack of peripheral B cells and serum IgM. These results were confirmed in mice containing the homozygous JH deletion in their germ line. This novel B-cell-deficient mouse strain provides a tool for studying the recombination and expression of exogenous immunoglobulin genes introduced into the mouse germ line.

摘要

利用最近描述的一种在胚胎干细胞中高效获得纯合靶向等位基因的方法，我们培育出了嵌合小鼠，其组织部分源自携带小鼠免疫球蛋白重链连接区（JH）纯合缺失的胚胎干细胞。对这些嵌合小鼠的特征分析表明，JH纯合缺失导致B细胞发育在早期阶段停滞，致使外周B细胞和血清IgM完全缺失。这些结果在种系中含有JH纯合缺失的小鼠中得到了证实。这种新型的B细胞缺陷小鼠品系为研究导入小鼠种系的外源免疫球蛋白基因的重组和表达提供了一种工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4947/46126/7e07b8167c90/pnas01465-0463-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4947/46126/04ed19812d73/pnas01465-0463-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4947/46126/7e07b8167c90/pnas01465-0463-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4947/46126/04ed19812d73/pnas01465-0463-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4947/46126/7e07b8167c90/pnas01465-0463-b.jpg

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