Mouthon L, Nobrega A, Nicolas N, Kaveri S V, Barreau C, Coutinho A, Kazatchkine M D
Institut National de la Santé et de la Recherche Médicale Unité 430, Hôpital Broussais, Paris, France.
Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3839-43. doi: 10.1073/pnas.92.9.3839.
Analysis of the reactivity of IgM with self-antigens in tissues by a quantitative immunoblotting technique showed striking invariance among newborns in the human and in the mouse. The self-reactive repertoire of IgM of adults was also markedly conserved; it comprised most anti-self reactivities that prevailed among neonates. Multivariate analysis confirmed the homogeneity of IgM repertoires of neonates toward self- and non-self-antigens. Multivariate analysis discriminated between newborn and adult repertoires for reactivity with two of five sources of self-proteins and with non-self-antigens. Our observations support the concept that naturally activated B lymphocytes are selected early in development and throughout life for reactivity with a restricted set of self-antigens.
通过定量免疫印迹技术分析组织中IgM与自身抗原的反应性，结果显示人类和小鼠新生儿之间具有显著的不变性。成人IgM的自身反应性库也明显保守；它包含了大多数在新生儿中占主导的抗自身反应性。多变量分析证实了新生儿IgM库对自身和非自身抗原反应性的同质性。多变量分析区分了新生儿和成人库对五种自身蛋白来源中的两种以及非自身抗原的反应性。我们的观察结果支持这样一种概念，即天然活化的B淋巴细胞在发育早期及整个生命过程中被选择与一组有限的自身抗原发生反应。
