Coffman R L, Weissman I L
Nature. 1981 Feb 19;289(5799):681-3. doi: 10.1038/289681a0.
T200, a major cell-surface glycoprotein on lymphoid cells, exists in several forms with different electrophoretic mobilities. These forms have been correlated with different classes of lymphoid cell. The smaller forms, with molecular weights (MWs) of congruent to 170,000 and 180,000 are found predominantly on T cells while the 220,000 MW form is associated with B cells. The polypeptide portions of each molecule may be identical or closely related as all three forms share the same allelic variations, and all reported herologous antisera and monoclonal antibodies to T200 precipitate all three forms. We report here a monoclonal antibody specific for the 220,000 MW form of T200 and show that it is expressed only on B cells and a subset of bone marrow cells which includes B cell precursors. We suggest that this form of the molecule be designated provisionally B220.
T200是淋巴细胞上一种主要的细胞表面糖蛋白，以几种具有不同电泳迁移率的形式存在。这些形式与不同类别的淋巴细胞相关。分子量（MW）约为170,000和180,000的较小形式主要存在于T细胞上，而分子量为220,000的形式与B细胞相关。每个分子的多肽部分可能相同或密切相关，因为所有三种形式都共享相同的等位基因变异，并且所有报道的针对T200的异源抗血清和单克隆抗体都会沉淀所有三种形式。我们在此报告一种对分子量为220,000的T200形式具有特异性的单克隆抗体，并表明它仅在B细胞和包括B细胞前体的一部分骨髓细胞上表达。我们建议将这种分子形式暂时命名为B220。
