Barrett T, Visser I K, Mamaev L, Goatley L, van Bressem M F, Osterhaust A D
AFRC Institute for Animal Health, Pirbright Laboratory, Surrey, United Kingdom.
Virology. 1993 Apr;193(2):1010-2. doi: 10.1006/viro.1993.1217.
The morbilliviruses recently isolated from two cetacean species in the North and Mediterranean Seas have been shown to differ from phocine distemper virus isolated from European seals using monoclonal antibodies. We have identified a "universal" morbillivirus primer set, based on highly conserved regions of the morbillivirus phosphoprotein (P) gene and used this to amplify a region surrounding the RNA editing site from all known members of the group. Sequence analysis of this region of the gene shows that the dolphin and porpoise viruses are related but quite different from all other members of the group, forming a distinct lineage more closely related to the ruminant morbilliviruses than to the carnivore viruses.
最近从北海和地中海的两种鲸类动物中分离出的麻疹病毒,已被证明与使用单克隆抗体从欧洲海豹中分离出的海豹瘟病毒不同。我们基于麻疹病毒磷蛋白(P)基因的高度保守区域,鉴定出一套“通用”的麻疹病毒引物,并以此扩增该病毒组所有已知成员RNA编辑位点周围的区域。对该基因区域的序列分析表明,海豚和鼠海豚病毒具有相关性,但与该病毒组的所有其他成员有很大不同,形成了一个独特的谱系,与反刍动物麻疹病毒的关系比与食肉动物病毒的关系更为密切。