Suzuki Y, Yin Y, Makino M, Kurimura T, Wakamiya N
Department of Pathology, Osaka Prefectural Institute of Public Health, Japan.
Biochem Biophys Res Commun. 1993 Mar 15;191(2):335-42. doi: 10.1006/bbrc.1993.1222.
A 912 bp bovine cDNA fragment encoding bovine conglutinin was amplified by the RT-PCR technique. cDNA clones encoding the bovine conglutinin were isolated from a bovine liver cDNA library using a specific probe obtained from the PCR product. These cDNAs carry an insert of 1113 bp coding for a protein of 371 amino acid residues with a signal peptide of 20 residues. The deduced amino acid sequence of cDNA agrees with that determined by conventional amino acid sequence analysis. Two polyadenylation signal sequences were detected in the DNA sequence downstream of the 3' end of the gene. Southern blot analysis of total bovine genomic DNA indicated that there is only one copy of the gene encoding bovine conglutinin. Northern blot analysis of bovine tissues showed that conglutinin mRNA of about 1.5 kb is expressed in the liver and also slightly in the lung.
采用逆转录聚合酶链反应（RT-PCR）技术扩增出一段912 bp的编码牛凝集素的牛cDNA片段。使用从PCR产物获得的特异性探针，从牛肝脏cDNA文库中分离出编码牛凝集素的cDNA克隆。这些cDNA携带一个1113 bp的插入片段，编码一个由371个氨基酸残基组成的蛋白质，其信号肽由20个残基组成。cDNA推导的氨基酸序列与通过传统氨基酸序列分析确定的序列一致。在该基因3'端下游的DNA序列中检测到两个聚腺苷酸化信号序列。对牛基因组总DNA的Southern印迹分析表明，编码牛凝集素的基因只有一个拷贝。对牛组织的Northern印迹分析表明，约1.5 kb的凝集素mRNA在肝脏中表达，在肺中也有少量表达。
