Mogensen M M, Tucker J B, Baggaley T B
School of Biological and Medical Sciences, University of St. Andrews, Fife, Scotland/United Kingdom.
Eur J Cell Biol. 1993 Feb;60(1):67-75.
Multiple plasma membrane-associated microtubule-organizing centers operate in the cone cells of Drosophila ommatidia. A transcellular array of about 250 microtubules assembles in each cone cell during late pupal ommatidial morphogenesis. While these arrays are assembling, cone cells do not possess conventional centriole-containing centrosomal microtubule-organizing centers. The microtubules are associated with plasma membrane-associated plaques at both the apical and basal surfaces. During assembly of the arrays there is a progressive decrease in the number of microtubules/cell cross section at successively lower levels in each cell which is indicative of apicobasal microtubule elongation. In this respect, assembly of the arrays closely resembles that of transcellular 15 protofilament microtubules in late pupal wing cells (Mogensen et al. J. Cell Biol. 108, 1445-1452 (1989)). However, cone cell microtubules are almost certainly of the 13 protofilament variety as found in eukaryotic cells generally. We suggest that plasma membrane-associated microtubule-organizing centers are widely employed in polarized epithelia in Drosophila during late pupal morphogenesis.
多个与质膜相关的微管组织中心在果蝇小眼的视锥细胞中发挥作用。在蛹后期小眼形态发生过程中，每个视锥细胞中会组装形成一个由约250根微管组成的跨细胞阵列。在这些阵列组装期间，视锥细胞不具备含有传统中心粒的中心体微管组织中心。微管与顶端和基底表面的质膜相关斑块相连。在阵列组装过程中，每个细胞中依次较低水平的微管/细胞横截面积逐渐减少，这表明微管从顶端到基底方向伸长。在这方面，阵列的组装与蛹后期翅细胞中跨细胞15原纤维微管的组装非常相似（莫根森等人，《细胞生物学杂志》108卷，1445 - 1452页（1989年））。然而，视锥细胞微管几乎肯定是真核细胞中普遍存在的13原纤维类型。我们认为，与质膜相关的微管组织中心在果蝇蛹后期形态发生过程中被广泛应用于极化上皮细胞中。
