胃动素和红霉素可刺激从豚鼠胃中分离出的主细胞分泌胃蛋白酶原。

Motilin and erythromycin stimulate pepsinogen secretion by chief cells isolated from guinea pig stomach.

作者信息

Fiorucci S, Morelli A

机构信息

Istituto di Gastroenterologia ed Endoscopia Digestiva, Università di Perugia, Italy.

出版信息

Gastroenterology. 1993 Apr;104(4):1030-6. doi: 10.1016/0016-5085(93)90270-m.

DOI:10.1016/0016-5085(93)90270-m

PMID:8462791

Abstract

BACKGROUND

Motilin and erythromycin bind the same receptor in the gastrointestinal muscle cells. Motilin stimulates pepsinogen secretion in humans and dogs. However, it is unclear whether it acts directly on gastric chief cells or via other neurotransmitters.

METHODS

Isolated gastric chief cells were obtained from guinea pig stomach by collagenase digestion and calcium chelation with ethylene glycol-bis(B-amynomethyl ether)-N,N,N',N'-tetraacetic acid.

RESULTS

Significant stimulation of pespinogen secretion induced by motilin occurred at 1 pmol/L, half maximum at 10 pmol/L, and maximum at 100 pmol/L. Erythromycin caused significant stimulation at 1 pmol/L, half maximum at 100 pmol/L, and maximum at 1 nmol/L. Atropine (1 mumol/L) had no effect on either motilin- or erythromycin-induced pepsinogen secretion. Motilin and erythromycin determined a threefold increase in the intracellular calcium concentrations. Pretreatment of isolated chief cells with motilin and erythromycin induced a reversible, dose- and time-dependent desensitization of the pepsinogen secretion stimulated by carbachol and cholecystokinin but not that stimulated by secretin, vasoactive intestinal peptide, or prostaglandin E2.

CONCLUSIONS

Motilin and erythromycin directly stimulate pepsinogen secretion in isolated chief cells.

摘要

背景

胃动素和红霉素在胃肠道肌肉细胞中结合相同的受体。胃动素可刺激人和犬的胃蛋白酶原分泌。然而，尚不清楚其是直接作用于胃主细胞还是通过其他神经递质发挥作用。

方法

通过胶原酶消化并用乙二醇双（β-氨基乙醚）-N，N，N'，N'-四乙酸进行钙螯合，从豚鼠胃中获取分离的胃主细胞。

结果

胃动素诱导胃蛋白酶原分泌的显著刺激在1 pmol/L时出现，半数最大效应浓度在10 pmol/L，最大效应浓度在100 pmol/L。红霉素在1 pmol/L时引起显著刺激，半数最大效应浓度在100 pmol/L，最大效应浓度在1 nmol/L。阿托品（1 μmol/L）对胃动素或红霉素诱导的胃蛋白酶原分泌均无影响。胃动素和红霉素使细胞内钙浓度增加了三倍。用胃动素和红霉素预处理分离的主细胞可诱导对卡巴胆碱和胆囊收缩素刺激的胃蛋白酶原分泌产生可逆的、剂量和时间依赖性脱敏，但对促胰液素、血管活性肠肽或前列腺素E2刺激的胃蛋白酶原分泌无此作用。