Nürnberg P, Thiel G, Weber F, Epplen J T
Institut für Medizinische Genetik, Charité, Berlin, Germany.
Hum Genet. 1993 Mar;91(2):190-2. doi: 10.1007/BF00222724.
The termini of human chromosomes comprise stretches of G-rich repeats that are about 5-20 kilobase (kb) in length. The size of the telomeres can be determined by hybridization with probes specific for these (ttaggg)n sequences after digestion of chromosomal DNA with appropriate restriction enzymes and electrophoretic separation of the fragments. Here, probing with the 32P-labelled synthetic (TTAGGG)3 oligonucleotide revealed length changes of the telomeres occurring in intracranial tumours. Among 60 samples, analysed, 41.7% showed telomere elongation, and 21.7% telomere reduction, whereas 36.7% of the tumours exhibited equal lengths compared with the patients' peripheral blood leukocytes. Most of the elongated glioma telomeres exceeded in length those of untransformed astrocytes derived from human fetal tissue.
人类染色体的末端包含长度约为5 - 20千碱基（kb）的富含G的重复序列。在使用适当的限制性内切酶消化染色体DNA并对片段进行电泳分离后，通过与针对这些（ttaggg）n序列的特异性探针杂交，可以确定端粒的大小。在此，用32P标记的合成（TTAGGG）3寡核苷酸进行探测，揭示了颅内肿瘤中端粒发生的长度变化。在分析的60个样本中，41.7%显示端粒延长，21.7%显示端粒缩短，而36.7%的肿瘤与患者外周血白细胞相比显示长度相等。大多数延长的胶质瘤端粒长度超过了源自人类胎儿组织的未转化星形胶质细胞的端粒长度。
