Moyzis R K, Buckingham J M, Cram L S, Dani M, Deaven L L, Jones M D, Meyne J, Ratliff R L, Wu J R
Genetics Group, LS-3, Los Alamos National Laboratory, University of California 87545.
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6622-6. doi: 10.1073/pnas.85.18.6622.
A highly conserved repetitive DNA sequence, (TTAGGG)n, has been isolated from a human recombinant repetitive DNA library. Quantitative hybridization to chromosomes sorted by flow cytometry indicates that comparable amounts of this sequence are present on each human chromosome. Both fluorescent in situ hybridization and BAL-31 nuclease digestion experiments reveal major clusters of this sequence at the telomeres of all human chromosomes. The evolutionary conservation of this DNA sequence, its terminal chromosomal location in a variety of higher eukaryotes (regardless of chromosome number or chromosome length), and its similarity to functional telomeres isolated from lower eukaryotes suggest that this sequence is a functional human telomere.
一种高度保守的重复DNA序列，(TTAGGG)n，已从人重组重复DNA文库中分离出来。与通过流式细胞术分选的染色体进行定量杂交表明，该序列在每个人类染色体上的含量相当。荧光原位杂交和BAL-31核酸酶消化实验均揭示了该序列在所有人类染色体端粒处的主要簇集。这种DNA序列的进化保守性、其在多种高等真核生物中的染色体末端定位（无论染色体数目或染色体长度如何）以及其与从低等真核生物中分离出的功能性端粒的相似性表明，该序列是功能性人类端粒。
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