Ferretti A, Flanagan V P
Lipids. 1977 Feb;12(2):198-203. doi: 10.1007/BF02533294.
This study, which deals with the distribution of hydrocarbons in seven types of rabbit tissues, was done for the purpose of providing information that might help shed light on the biological relevance of the hydrocarbons in mammalian metabolism. Liver, kidneys, brain, spleen, skeletal muscle, perinephric adipose, and a sample of blood serum were collected from a single animal for analysis of their hydrocarbon composition. The analytical methodology consisted of solvent extraction, saponification (adipose), elution chromatography on hydrated alumina, and combined gas chromatography-mass spectrometry. Hydrocarbons were detected in all of the tissues examined at concentrations estimated to range from 0.1 to 0.01% of the total lipid extracted Three quite distinct distribution modes were recognized. The bulk of the identified components consisted of normal, saturated, nonterpenoid hydrocarbons in the C16 to C33 range. Squalene, phytene, phytadiene, and pristane were the only terpenoids detected. Nonterpenoid branched (iso and anteiso) hydrocarbons were identified unequivocally and in significant amounts in the muscle only. The adipose was the only tissue which was relatively rich in monoalkenes, and its overall hydrocarbon composition closely resembled that of the feed. The results of the study are not consistent with metabolic inertness. The observed qualitative and quantitative differences might reflect function and metabolic activities of the individual organs in a way yet to be elucidated.
本研究针对七种兔组织中碳氢化合物的分布展开，目的是提供有助于阐明碳氢化合物在哺乳动物新陈代谢中的生物学相关性的信息。从一只动物身上采集了肝脏、肾脏、大脑、脾脏、骨骼肌、肾周脂肪和一份血清样本，用于分析其碳氢化合物组成。分析方法包括溶剂萃取、皂化（脂肪）、在水合氧化铝上进行洗脱色谱以及气相色谱 - 质谱联用。在所检测的所有组织中均检测到了碳氢化合物，其浓度估计占提取的总脂质的0.1%至0.01%。识别出了三种截然不同的分布模式。所鉴定的成分大部分由碳数在C16至C33范围内的正构、饱和、非萜类碳氢化合物组成。角鲨烯、植烯、植二烯和姥鲛烷是仅检测到的萜类化合物。非萜类支链（异和反异）碳氢化合物仅在肌肉中被明确鉴定出且含量显著。脂肪是唯一相对富含单烯烃且其整体碳氢化合物组成与饲料极为相似的组织。该研究结果与代谢惰性不一致。所观察到的定性和定量差异可能以一种尚待阐明的方式反映了各个器官的功能和代谢活动。
