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未萌出小牛牙齿牙髓中的甘油酯水解酶活性。

Glycerol ester hydrolase activity in the pulp of unerupted calves' teeth.

作者信息

McMahon K E, Pitas R E, Jensen R G

出版信息

Lipids. 1977 Feb;12(2):238-9. doi: 10.1007/BF02533302.

DOI:10.1007/BF02533302
PMID:846310
Abstract

The pulp from unerupted calves' teeth was found to contain low levels of glycerol ester hydrolase activity. Solutions in Tris buffer cleared tributyrylglycerol agar and hydrolyzed emulsified olive oil. The average quantity of free fatty acids released in five assays with olive oil was: 2.8, S.D.+/-1.4. Specific activities were: 0.25, S.D.+/-0.13, mkatals/kg protein.

摘要

未萌出小牛牙齿的牙髓中甘油酯水解酶活性水平较低。在Tris缓冲液中的溶液能使三丁酰甘油琼脂澄清并水解乳化橄榄油。五次橄榄油测定中释放的游离脂肪酸平均量为:2.8,标准差±1.4。比活性为:0.25,标准差±0.13,mkatals/千克蛋白质。

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引用本文的文献

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Detection and determination of lipase (acylglycerol hydrolase) activity from various sources.检测和测定来自各种来源的脂肪酶(酰基甘油水解酶)活性。
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本文引用的文献

1
Determination of serum proteins by means of the biuret reaction.通过双缩脲反应测定血清蛋白。
J Biol Chem. 1949 Feb;177(2):751-66.
2
Purification of triglycerides with an alumina column.
Lipids. 1966 Nov;1(6):451-2. doi: 10.1007/BF02532552.
3
Lipid determination in powdered human dentin by thin-layer and gas-liquid chromatography.采用薄层色谱法和气液色谱法测定人牙本质粉末中的脂质。
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Phylogeny of lipase specificity.脂肪酶特异性的系统发育。
Lipids. 1970 Jun;5(6):558-62. doi: 10.1007/BF02532745.
5
Tributyrinase activity of leptospires: fixed and soluble tributyrinase demonstrated by means of an agar diffusion test.钩端螺旋体的丁酸酯酶活性:通过琼脂扩散试验证明的固定型和可溶性丁酸酯酶
Acta Pathol Microbiol Scand. 1965;65(2):259-70. doi: 10.1111/apm.1965.65.2.259.
6
Characterization, subcellular localization, and partial purification of a heparin-released triglyceride lipase from rat liver.大鼠肝脏中肝素释放的甘油三酯脂肪酶的特性、亚细胞定位及部分纯化
J Biol Chem. 1973 Mar 25;248(6):1992-9.
7
Effects of dietary restriction on the composition of lipids in rat teeth.饮食限制对大鼠牙齿脂质成分的影响。
Arch Oral Biol. 1975 Feb;20(2):131-5. doi: 10.1016/0003-9969(75)90167-3.