Najab-Benhayoun M, Serne H, Jozefowicz M, Fischer A M, Brisson C, Sultan Y
Laboratoire de Recherches sur les Macromolécules, CNRS-URA 502, Université Paris-Nord, Villetaneuse, France.
J Biomed Mater Res. 1993 Apr;27(4):511-20. doi: 10.1002/jbm.820270412.
Biospecific functional polymers, i.e., polymers randomly substituted with specific chemical functional groups, were designed to interact with living systems. Interactions between polystyrene sodium sulfonate (PSSO3Na) and insulin secreting RINm5F cells have been previously described. For the sake of comparison, interactions of PSSO3Na with human umbilical vein endothelial cells (HUVEC) were studied. In this case, the interaction is indirect, i.e., mediated by a binding protein, fibronectin (Fn). This was evidenced by HUVEC culture on Fn precoated PSSO3Na microcarriers. The interactions between PSSO3Na and HUVEC result in a biologically normal proliferation of cells and synthesis and secretion of Von Willebrand Factor (VWF). These results show that different biospecific interactions may occur between cells in culture, binding proteins and polymers randomly substituted with suitable functional groups. HUVEC, when cultured on heparin-like microcarriers, behave differently from other cells like RINm5F, whose interaction with the same polymers is not mediated by binding proteins.
生物特异性功能聚合物，即随机被特定化学官能团取代的聚合物，被设计用于与生物系统相互作用。聚苯乙烯磺酸钠（PSSO3Na）与分泌胰岛素的RINm5F细胞之间的相互作用此前已有描述。为作比较，研究了PSSO3Na与人脐静脉内皮细胞（HUVEC）的相互作用。在这种情况下，相互作用是间接的，即由一种结合蛋白纤连蛋白（Fn）介导。这在Fn预包被的PSSO3Na微载体上培养HUVEC得到了证实。PSSO3Na与HUVEC之间的相互作用导致细胞正常增殖以及血管性血友病因子（VWF）的合成与分泌。这些结果表明，在培养的细胞、结合蛋白与随机被合适官能团取代的聚合物之间可能会发生不同的生物特异性相互作用。当HUVEC在类肝素微载体上培养时，其行为与RINm5F等其他细胞不同，RINm5F与相同聚合物的相互作用不是由结合蛋白介导的。
