Laboratoire de Bio ingénierie et Biomécanique Ostéoarticulaires, UMR CNRS 7052, 10, Avenue de Verdun, 75010, Paris, France.
J Mater Sci Mater Med. 2013 Jul;24(7):1745-54. doi: 10.1007/s10856-013-4932-3. Epub 2013 Apr 27.
Titanium surface modifications to simultaneously prevent bacterial adhesion but promote bone-cell functions could be highly beneficial for improving implant osseointegration. In the present in vitro study, the effect of sulfonate groups on titanium surfaces was investigated with respect to both S. aureus adhesion and osteoblast functions pertinent to new bone formation. Commercial pure titanium (cpTi) squares were oxydized (Tiox), grafted with poly(sodium styrene sulfonate) groups (Tigraft) by covalent bonding using radical polymerization, and were characterized by infrared spectroscopy (HATR-FTIR) and colorimetry. Bacterial adhesion study showed that Tigraft exhibited high inhibition of S. aureus adhesion S at levels >90 %, when compared to cpTi (P < 0.05). In contrast osteoblasts adhesion was similar on all three titanium surfaces. While the kinetics of cell proliferation were similar on the three titanium surfaces, Alkaline phosphatase-specific activity of osteoblasts cultured on Tigraft surfaces was twofold higher than that observed on either on Tiox or cpTi surfaces (P < 0.01). More importantly, the amount and the distribution of calcium-containing nodules was different. The total area covered by calcium-containing nodules was 2.2-fold higher on the Tigraft as compared to either Tiox or cpTi surfaces (P < 0.01). These results provide evidence that poly(sodium styrene sulfonate) groups grafting on cpTi simultaneously inhibits bacteria adhesion but promote osteoblast function pertinent to new bone formation. Such modified titanium surfaces offer a promising strategy for preventing biofilm-related infections and enhancing osteointegration of implants in orthopaedic and dental applications.
钛表面改性可同时防止细菌黏附，促进骨细胞功能，这对改善种植体骨整合具有重要意义。本体外研究探讨了磺酸基团对金黄色葡萄球菌黏附及与新骨形成相关的成骨细胞功能的影响。采用自由基聚合，通过共价键将聚（苯乙烯磺酸钠）基团接枝到商业纯钛（cpTi）方块上（Tigraft），并通过红外光谱（HATR-FTIR）和比色法进行了表征。细菌黏附研究表明，与 cpTi 相比，Tigraft 对金黄色葡萄球菌黏附的抑制率>90%，具有显著差异（P<0.05）。然而，三种钛表面的成骨细胞黏附率相似。虽然三种钛表面的细胞增殖动力学相似，但在 Tigraft 表面培养的成骨细胞碱性磷酸酶的特异性活性是 Tiox 或 cpTi 表面的两倍（P<0.01）。更为重要的是，钙结节的数量和分布不同。Tigraft 表面钙结节的总面积比 Tiox 或 cpTi 表面高 2.2 倍（P<0.01）。这些结果表明，将聚（苯乙烯磺酸钠）基团接枝到 cpTi 上可同时抑制细菌黏附，促进与新骨形成相关的成骨细胞功能。这种改性钛表面为预防生物膜相关感染和增强骨科和牙科应用中植入物的骨整合提供了一种有前景的策略。
