Battye K M, Parkinson T J, Jenner L J, Evans G, O'Neill C, Lamming G E
Department of Physiology, University of Sydney, Royal North Shore Hospital, St Leonards, NSW, Australia.
J Reprod Fertil. 1993 Jan;97(1):21-6. doi: 10.1530/jrf.0.0970021.
In sheep, the presence of an embryo in utero on the 12th to 13th day after oestrus prevents luteolysis. These studies investigated whether platelet-activating factor (PAF) could exert an antiluteolytic function, either alone or in combination with interferon. The intrauterine administration of 250 micrograms PAF per horn per day, administered through indwelling cannulae into the uterus as injections twice a day (n = 12) or by continuous infusion (n = 4) failed to extend luteal function compared with controls (n = 8). When indwelling cannulae were used to administer (i) 125 micrograms PAF per uterine horn, as a bolus infusion twice a day (n = 5), (ii) continuous infusion of 500 micrograms bovine recombinant alpha 1-interferon each day (brIFN, n = 5), (iii) 125 micrograms PAF per horn twice a day, plus 500 micrograms brIFN per day (n = 8), or (iv) vehicle (n = 5), the luteal phase was significantly longer in co-infused (iii) than in control (iv) animals. These findings indicate that pharmacological doses of PAF may act synergistically with interferons to prevent luteolysis.
在绵羊中，发情后第12至13天子宫内存在胚胎可防止黄体溶解。这些研究调查了血小板激活因子（PAF）是否能单独或与干扰素联合发挥抗黄体溶解作用。通过留置套管每天向每个子宫角注射250微克PAF（每天两次，n = 12）或持续输注（n = 4），与对照组（n = 8）相比，未能延长黄体功能。当使用留置套管给药时：（i）每个子宫角以大剂量输注125微克PAF，每天两次（n = 5）；（ii）每天持续输注500微克牛重组α1干扰素（brIFN，n = 5）；（iii）每个子宫角每天两次注射125微克PAF，加每天500微克brIFN（n = 8）；或（iv）赋形剂（n = 5），联合输注组（iii）动物的黄体期明显长于对照组（iv）动物。这些发现表明，药理学剂量的PAF可能与干扰素协同作用以防止黄体溶解。
