Martal J, Degryse E, Charpigny G, Assal N, Reinaud P, Charlier M, Gaye P, Lecocq J P
I.N.R.A. Unité Endocrinologie de l'Embryon, Jouy en Josas, France.
J Endocrinol. 1990 Dec;127(3):R5-8. doi: 10.1677/joe.0.127r005.
Ovine trophoblastin (oTP) is a natural interferon of the class-II interferon-alpha subfamily. Recombinant ovine trophoblastin (r.oTP), produced by genetic engineering, was purified by anion-exchange HPLC. The product exhibited a high degree of homogeneity (greater than 98%), and similar immunological cross reaction and antiviral activity to natural oTP. Antiluteolytic activity of r.oTP was established by intrauterine injection in two groups of cyclic recipient ewes. Control group A included 10 ewes which received sterile BSA in saline twice daily for 8 days (from day 10-12 of oestrous cycle). Experimental group B included 17 ewes which received 80 micrograms (4 ewes), 170 micrograms (8 ewes) or 340 micrograms (5 ewes) r.oTP daily for 8 days. Maintenance of functional corpora lutea for 1 month or more was observed in 4 out of 5 ewes which received high doses of r.oTP. These results indicate that oTP alone extends luteal secretory activity.
绵羊滋养层蛋白（oTP）是II类干扰素α亚家族的一种天然干扰素。通过基因工程生产的重组绵羊滋养层蛋白（r.oTP），经阴离子交换高效液相色谱法纯化。该产品显示出高度的均一性（大于98%），并且与天然oTP具有相似的免疫交叉反应和抗病毒活性。通过对两组发情期受体母羊进行子宫内注射，确定了r.oTP的抗黄体溶解活性。对照组A包括10只母羊，每天两次在盐水中接受无菌牛血清白蛋白，共8天（发情周期的第10 - 12天）。实验组B包括17只母羊，每天分别接受80微克（4只母羊）、170微克（8只母羊）或340微克（5只母羊）的r.oTP，共8天。在接受高剂量r.oTP的5只母羊中，有4只观察到功能性黄体维持1个月或更长时间。这些结果表明，单独使用oTP可延长黄体分泌活性。
