Miyakawa H, Abe K, Nakamura H, Nagai K, Kako M, Furue H
4th Department of Internal Medicine, Teikyo University, School of Medicine.
Nihon Rinsho. 1993 Feb;51(2):292-6.
To clarify the relationship between a point mutation of HBV-DNA Pre-C region and the serological feature of chronic hepatitis B, we determined the Pre-C region sequence of HBV-DNA obtained sera of 39 patients with chronic hepatitis B. The screening of the gene arrangement of Pre-C region of HBV-DNA was performed by a direct sequence method amplifying HBV-DNA by polymerase chain reaction methods. In 22 of HBeAg positive cases, a mutant type (the 28th codon changed from TGA to TAG: stop codon) was found in only one case. The other hand, In 17 of HBeAg negative cases, it was found in 6 cases with fluctuating ALT and DNA-polymerase levels. We concluded that mutant viral infections could be the main cause of HBeAg negative cases with fluctuating ALT and DNA-polymerase levels.
为阐明乙肝病毒（HBV）DNA前C区点突变与慢性乙型肝炎血清学特征之间的关系，我们测定了39例慢性乙型肝炎患者血清中HBV-DNA的前C区序列。采用聚合酶链反应方法扩增HBV-DNA，通过直接测序法对HBV-DNA前C区的基因排列进行筛查。在22例HBeAg阳性病例中，仅1例发现突变型（第28密码子由TGA变为TAG：终止密码子）。另一方面，在17例HBeAg阴性病例中，6例ALT和DNA聚合酶水平波动者发现有该突变。我们得出结论，突变病毒感染可能是ALT和DNA聚合酶水平波动的HBeAg阴性病例的主要原因。
