Pallikaris I G, Tslimbaris M K, Iliaki O E, Naoumidi I I, Georgiades A, Panagopoulos I A
Department of Ophthalmology, University of Crete Medical School, Greece.
Lasers Surg Med. 1993;13(2):197-203. doi: 10.1002/lsm.1900130207.
We used chloroaluminum sulfonated phthalocyanine as a photo-sensitizer and a diode laser as a light source for induction of photothrombosis of corneal neovascularization. Corneal neovascularization was induced in 1 eye of each of 10 New Zealand white rabbits using intrastromal 6.0 silk sutures. After the intravenous injection of phthalocyanine in a dose of 4 mg per kg of body weight, photothrombosis was carried out using a diode laser emitting at 675 nm. The animals were followed up by both fluorescein angiography and slit-lamp photography for up to three months. At the end of the follow-up period the eyes were prepared for histology. After photothrombosis, thrombus formation was induced within new vessels demonstrating histological characteristics of both arterioles and venules. Most of the vessels disappeared or remained closed during the follow-up period. Recanalization of some of the thrombosed vessels occurred within the first 20 days after the operation. The combination of phthalocyanine and a 675 nm diode laser is effective for the induction of photothrombosis of corneal neovascularization.
我们使用氯铝磺化酞菁作为光敏剂，并用二极管激光器作为光源来诱导角膜新生血管形成的光血栓形成。使用基质内6.0丝线缝合，在10只新西兰白兔的每只兔子的一只眼睛中诱导角膜新生血管形成。在静脉注射剂量为每公斤体重4毫克的酞菁后，使用发射波长为675纳米的二极管激光器进行光血栓形成。通过荧光素血管造影和裂隙灯摄影对动物进行长达三个月的随访。在随访期结束时，将眼睛准备用于组织学检查。光血栓形成后，在新血管内诱导血栓形成，显示出小动脉和小静脉的组织学特征。在随访期间，大多数血管消失或保持闭合。术后头20天内，一些血栓形成的血管发生了再通。酞菁与675纳米二极管激光器联合使用对诱导角膜新生血管形成的光血栓形成有效。
