Tsilimbaris M K, Naoumidi I I, Naoumidis L P, Papageorgiou E, Pallikaris I G
Vardinoyannion Eye Institute of Crete, Department of Ophthalmology, School of Health Sciences, University of Crete, Greece.
Ophthalmic Surg Lasers. 1997 Jun;28(6):483-94.
To evaluate the morphologic and functional effects of ciliary body photodynamic therapy (PDT) using phthalocyanine and a diode laser.
The upper half of the left eye ciliary body of 16 albino rabbits was irradiated transsclerally using a 670-nm diode laser (400 mW/cm2) after intravenous injection of phthalocyanine (6 mg/kg). The animals were observed for a maximum of 2 months by means of tonometry, biomicroscopy, and fundus examination. At the end of the follow-up period, they were killed and their eyes were prepared for light and electron microscopy.
Transscleral PDT resulted in intraocular pressure (IOP) reduction in the treated eye, which lasted about 2 weeks. During this time, the treated eye had IOP values that were significantly lower than its baseline IOP values and the IOP values of the untreated eye (P < .05). One month after the procedure, the IOP had returned to baseline values. Histologic examination revealed vascular endothelial cell damage causing vascular thrombosis in the treated areas. The architecture of the two ciliary epithelium layers showed a significant abnormality. Disappearance of epithelial apical junction complexes and loss of the normal b-cytomembrane enfolding were observed in the course of electron microscopic examination. Large intercellular spaces between epithelial cells were noticed. All of these changes had subsided by the end of the second postoperative month.
Transscleral phthalocyanine-mediated PDT with the parameters used in this experiment results in significant but temporary functional and morphologic alterations in the ciliary bodies of albino rabbits.
评估使用酞菁和二极管激光进行睫状体光动力疗法（PDT）的形态学和功能效果。
给16只白化兔静脉注射酞菁（6mg/kg）后，使用670nm二极管激光（400mW/cm²）经巩膜照射左眼睫状体上半部分。通过眼压测量、生物显微镜检查和眼底检查对动物进行最长2个月的观察。随访期结束时，将它们处死并对其眼睛进行光镜和电镜检查准备。
经巩膜PDT导致治疗眼眼压降低，持续约2周。在此期间，治疗眼的眼压值显著低于其基线眼压值和未治疗眼的眼压值（P<.05）。术后1个月，眼压恢复到基线值。组织学检查显示血管内皮细胞损伤导致治疗区域血管血栓形成。两层睫状体上皮的结构出现明显异常。在电镜检查过程中观察到上皮顶端连接复合体消失以及正常b细胞膜折叠丧失。注意到上皮细胞之间有大的细胞间隙。所有这些变化在术后第二个月末均已消退。
本实验所采用参数的经巩膜酞菁介导的PDT导致白化兔睫状体出现显著但暂时的功能和形态学改变。
