Kamiya Y, Ohmura E, Murakami H, Shizume K, Tsushima T, Demura H, Sakuma N
Department of Internal Medicine 3 Nagoya City University Medical School, Japan.
Life Sci. 1993;52(16):1381-6. doi: 10.1016/0024-3205(93)90173-z.
Transforming growth factor-alpha (TGF-alpha) in pleural and peritoneal effusions was assayed by homologous radioimmunoassay (RIA) and radioreceptor assay (RRA) using human placental membrane. Effusions were obtained from 24 patients with and 17 patients without cancer. Most of the effusions were found to contain TGF-alpha by RIA and RRA, but immunoreactive epidermal growth factor (EGF) was not detected. Effusions were chromatographed on Bio-Gel P-60 with several peaks of TGF-alpha activity by both RIA and RRA. A discrepancy in the chromatographic pattern of TGF-alpha between RIA and RRa suggested the existence of EGF-like substances capable of binding to EGF receptors which lack immunoreactivity for EGF or TGF-alpha. The TGF-alpha concentration of the acetic acid-extracted malignant effusions assayed by RRA significantly exceeded the value obtained from benign effusions (17.0 +/- 8.7 vs. 9.2 +/- 6.3 ng/ml; mean +/- SD: p < 0.02). However, the concentrations obtained by RIA did not differ.
采用同源放射免疫分析法（RIA）和放射受体分析法（RRA），利用人胎盘膜对胸腔积液和腹腔积液中的转化生长因子-α（TGF-α）进行检测。积液取自24例癌症患者和17例非癌症患者。通过RIA和RRA发现，大多数积液中含有TGF-α，但未检测到免疫反应性表皮生长因子（EGF）。积液在Bio-Gel P-60上进行色谱分析，RIA和RRA均显示有几个TGF-α活性峰。RIA和RRa之间TGF-α色谱图的差异表明存在能够与EGF受体结合的EGF样物质，这些物质对EGF或TGF-α缺乏免疫反应性。通过RRA检测，乙酸提取的恶性积液中TGF-α浓度显著超过良性积液（17.0±8.7对9.2±6.3 ng/ml；均值±标准差：p<0.02）。然而，RIA获得的浓度没有差异。
