Ito E, Toki T, Ishihara H, Ohtani H, Gu L, Yokoyama M, Engel J D, Yamamoto M
Department of Pediatrics, Hirosaki University School of Medicine, Japan.
Nature. 1993 Apr 1;362(6419):466-8. doi: 10.1038/362466a0.
The transcription factor GATA-1 is a fundamental regulator of genes in haematopoietic cell lineages and belongs to a family of factors that bind to the consensus sequence WGATAR. The GATA motif was originally identified in cis-regulatory regions of globin and other erythroid-specific genes, but the range of genes controlled by the GATA factors has since expanded. Members of the GATA transcription factor family share a conserved zinc-finger DNA-binding domain, but the expression profile of each GATA factor is distinct. Here we show that a testis form of murine (m)GATA-1 messenger RNA is transcribed from a promoter located 5' to the erythroid first exon, and the remaining exons (which encode the mGATA-1 protein) are used in common by both testis and erythroid transcripts. We use an anti-mGATA-1 monoclonal antibody to show that the factor expressed in erythroid cells is the same as that found in the seminiferous tubules of murine testis. The GATA-1-expressing cells in 10-week-old testis were found only in contact with the basement membrane of seminiferous tubules, suggesting that GATA-1 regulates genes during the earliest stages of spermatogenesis.
转录因子GATA-1是造血细胞谱系中基因的基本调节因子，属于与共有序列WGATAR结合的因子家族。GATA基序最初是在珠蛋白和其他红系特异性基因的顺式调节区域中鉴定出来的，但此后由GATA因子控制的基因范围有所扩大。GATA转录因子家族的成员共享一个保守的锌指DNA结合结构域，但每个GATA因子的表达谱是不同的。在这里，我们表明，小鼠（m）GATA-1信使核糖核酸的睾丸形式是从位于红系第一个外显子5'端的启动子转录而来的，其余外显子（编码mGATA-1蛋白）被睾丸和红系转录本共同使用。我们使用抗mGATA-1单克隆抗体来表明，在红系细胞中表达的因子与在小鼠睾丸生精小管中发现的因子相同。在10周龄睾丸中表达GATA-1的细胞仅在与生精小管的基底膜接触处被发现，这表明GATA-1在精子发生的最早阶段调节基因。
