百日咳毒素分泌所需操纵子的分子特征分析。
Molecular characterization of an operon required for pertussis toxin secretion.
作者信息
Weiss A A, Johnson F D, Burns D L
机构信息
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298.
出版信息
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2970-4. doi: 10.1073/pnas.90.7.2970.
Abstract
Mutants of Bordetella pertussis which are defective in secretion of pertussis toxin were isolated and characterized. The region of the B. pertussis chromosome identified by mutagenesis as playing a role in transport of pertussis toxin was sequenced. Analysis of this region revealed eight open reading frames, seven of which predict a protein exhibiting homology with one of the VirB proteins of Agrobacterium tumefaciens, which are involved in the transport of the T-DNA molecule across bacterial and plant membranes. Thus a set of accessory proteins are most likely involved in the secretion of pertussis toxin, and these proteins appear to be members of a family of proteins involved in the secretion of macromolecules from bacteria.
摘要
分离并鉴定了百日咳博德特氏菌中百日咳毒素分泌缺陷的突变体。对通过诱变确定在百日咳毒素转运中起作用的百日咳博德特氏菌染色体区域进行了测序。对该区域的分析揭示了八个开放阅读框，其中七个预测的蛋白质与根癌土壤杆菌的一种VirB蛋白具有同源性，这些蛋白参与T-DNA分子跨细菌和植物膜的转运。因此，一组辅助蛋白很可能参与百日咳毒素的分泌，并且这些蛋白似乎是参与细菌大分子分泌的蛋白家族的成员。
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