一种活动的人类精子头部固定方法。
A motile human sperm head fixation method.
作者信息
Ou M C, Ng H T, Chiang B N, Hong C Y, Hsu C T
机构信息
Institute of Clinical Medicine, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
出版信息
Andrologia. 1993 Mar-Apr;25(2):67-70. doi: 10.1111/j.1439-0272.1993.tb02684.x.
In a solution containing less than 5% seminal fluid, human sperm has a tendency to attach its head on the smooth surface of an object. Based on such a phenomenon, a simple method was developed to fix sperm head on the surface of glass slide. This method was demonstrated by studying caffeine effect on the tail movement of human sperm in phosphate buffered saline. By analysing the images recorded on a videocassette, caffeine, a well known stimulator of human sperm motility, was shown to induce an immediate increase of sperm TBF (tail beat frequency) and the amplitude of TBF increase was found to be larger in sperm with lower basal TBF value. This method can provide a flock of head-fixed motile sperm for a precise sperm tail movement assessment or for other purposes.
在精液含量低于5%的溶液中，人类精子倾向于将头部附着在物体的光滑表面上。基于这一现象，开发了一种将精子头部固定在载玻片表面的简单方法。通过研究咖啡因对磷酸盐缓冲盐水中人类精子尾部运动的影响，验证了该方法。通过分析录像带上记录的图像，发现咖啡因这种众所周知的人类精子活力刺激剂能立即引起精子尾部摆动频率（TBF）增加，且基础TBF值较低的精子中TBF增加的幅度更大。该方法可为精确评估精子尾部运动或其他目的提供一群头部固定的活动精子。
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