肝脏中三种类MEKA蛋白的纯化与鉴定：一种94 kDa蛋白与G蛋白βγ亚基的关联
Purification and characterization of three MEKA-like proteins in liver: association of a 94 kDa protein with beta gamma subunits of G-proteins.
作者信息
Kuo C H, Taira E, Takaha N, Sohma H, Akino T, Fukada Y, Sanada K, Miki N
机构信息
Department of Pharmacology, Osaka University Medical School, Japan.
出版信息
Biochem Biophys Res Commun. 1993 Mar 31;191(3):1097-104. doi: 10.1006/bbrc.1993.1329.
Retinal 32 kDa MEKA protein (rMEKA) exists in the photoreceptor cells and forms a complex with beta gamma subunit of transducin. Bovine liver contained three MEKA-like proteins (94 kDa, 35 kDa-a, 35 kDa-b) which reacted with a rMEKA antibody. Each protein was purified as a single band on a SDS-PAGE and used for a reconstitution experiment with alpha and beta gamma subunits of cerebral G-proteins (Go/i). The 94 kDa protein inhibited GTP-binding ability of G alpha by forming a complex with beta gamma subunit.
视网膜32千道尔顿MEKA蛋白（rMEKA）存在于光感受器细胞中，并与转导蛋白的βγ亚基形成复合物。牛肝中含有三种与rMEKA抗体反应的MEKA样蛋白（94千道尔顿、35千道尔顿-a、35千道尔顿-b）。每种蛋白在SDS-PAGE上均以单一条带形式纯化，并用于与脑G蛋白（Go/i）的α和βγ亚基进行重组实验。94千道尔顿的蛋白通过与βγ亚基形成复合物来抑制Gα的GTP结合能力。
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