Masumoto A, Yamamoto N
Department of Functional Morphology, School of Nursing Kitasato University, Kanagawa, Japan.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):1218-23. doi: 10.1006/bbrc.1993.1347.
Hepatocyte growth factor (HGF) purified from human placenta was specifically bound to a component in ECM secreted from lung fibroblasts, hepatocytes, and nonparenchymal liver cells. HGF was also bound to a component in basement membrane matrigel. The rate of DNA synthesis in hepatocytes cultured on the HGF-bound ECM was 4-7 times that of control hepatocytes. When ECM-coated dishes were pretreated with heparinase and heparitinase prior to binding of HGF, the stimulation decreased remarkably. A considerable decrease in the stimulation was observed following the washing of HGF-bound ECM with 1 M NaCl. We propose, based on these observations, that HGF bound to the ECM component present in Disse's space functions in regeneration following hepatic injury.
从人胎盘中纯化的肝细胞生长因子（HGF）特异性结合于肺成纤维细胞、肝细胞和肝非实质细胞分泌的细胞外基质（ECM）中的一种成分。HGF也结合于基底膜基质胶中的一种成分。在与HGF结合的ECM上培养的肝细胞中DNA合成速率是对照肝细胞的4至7倍。在用HGF结合之前，用肝素酶和硫酸乙酰肝素酶预处理ECM包被的培养皿，刺激作用显著降低。用1M NaCl洗涤与HGF结合的ECM后，观察到刺激作用明显下降。基于这些观察结果，我们提出，结合于狄氏间隙中存在的ECM成分的HGF在肝损伤后的再生中发挥作用。
