Suwa M, Mitaku S, Kuroda Y
Tokyo University of Agriculture and Technology, Faculty of Technology, Japan.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):782-9. doi: 10.1006/bbrc.1993.1285.
Human muscular dystrophy, the fatal disease, is caused by the genetic abnormality of dystrophin. The question whether dystrophin is an intrinsic membrane protein or not was investigated by calculating the average value and the long periodicity of hydrophobicity of amino acid sequence. The periodicity was estimated by a maximum entropy method of Fourier transformation. The results indicated that a fragment from 3101-st to 3200-th residues of dystrophin contains several transmembrane helices. The hydropathy plot of this region strongly suggests four transmembrane helices, indicating that both ends, N-and C-termini, are located in the cytoplasmic sides with firm anchoring into membrane by these helices.
人类肌肉萎缩症是一种致命疾病，由肌营养不良蛋白的基因异常引起。通过计算氨基酸序列疏水性的平均值和长周期，研究了肌营养不良蛋白是否为内在膜蛋白这一问题。周期通过傅里叶变换的最大熵方法进行估计。结果表明，肌营养不良蛋白3101至3200位残基的片段包含几个跨膜螺旋。该区域的亲水性图谱强烈提示有四个跨膜螺旋，表明其N端和C端都位于细胞质侧，并通过这些螺旋牢固地锚定在膜中。
