Kamimura Y, Sawada N, Aoki M, Mori M
Department of Pathology, Sapporo Medical College, Japan.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):817-22. doi: 10.1006/bbrc.1993.1290.
To examine the effects of endothelin-1(ET-1) on bile canalicular contractions, rat hepatocytes were isolated by a collagenase perfusion technique and cultured on p-n-p-vinylbenzenyl-D-lactonamide-coated dishes under serum-free conditions. The frequency of bile canalicular contractions was expressed by the percentage of the number of bile canaliculi(BC) that contracted compared to the total number of BC observed for 10 min. The treatment of the hepatocytes with ET-1 increased the frequency of contraction of the BC in a dose-dependent manner up to 2nM, control: 10.4%; 0.5nM ET-1:31.6%; 1nM ET-1:54.0%; 2nM ET-1:58.0%; 4nM ET-1:58.9%. The contraction, once it occurred, lasted for rest of the observation period. We also observed a transient increase in the concentration of intracellular Ca in cultured hepatocytes upon the addition of ET-1 to the medium. These results suggest that ET-1 plays an important role in the mortility of BC in vivo.
为研究内皮素-1（ET-1）对胆小管收缩的影响，采用胶原酶灌注技术分离大鼠肝细胞，并在无血清条件下培养于对硝基苯基-D-内酯酰胺包被的培养皿中。胆小管收缩频率以收缩的胆小管（BC）数量占观察10分钟内BC总数的百分比表示。用ET-1处理肝细胞，BC收缩频率呈剂量依赖性增加，直至2nM，对照组：10.4%；0.5nM ET-1：31.6%；1nM ET-1：54.0%；2nM ET-1：58.0%；4nM ET-1：58.9%。收缩一旦发生，在剩余观察期内持续存在。我们还观察到，向培养基中添加ET-1后，培养的肝细胞内Ca浓度短暂升高。这些结果表明，ET-1在体内BC的运动中起重要作用。
