Ryu S, Labbé R G
Department of Food Science, University of Massachusetts, Amherst.
Int J Food Microbiol. 1993 Feb;17(4):343-8. doi: 10.1016/0168-1605(93)90204-t.
Sporulating and vegetative cell extracts of enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens were examined by Western blotting using enterotoxin antiserum. A 48-kDa enterotoxin-related protein was found in vegetative and sporulating cell extracts of both toxin types. The results question previous reports about the ability of vegetative cells and presumptive enterotoxin-negative strains of this organism to produce enterotoxin, a protein with a molecular weight of 34 kDa.
使用肠毒素抗血清通过蛋白质免疫印迹法检测产气荚膜梭菌产肠毒素阳性和产肠毒素阴性菌株的芽孢形成细胞提取物和营养细胞提取物。在两种毒素类型的营养细胞和芽孢形成细胞提取物中均发现了一种48 kDa的肠毒素相关蛋白。这些结果对先前关于该生物体的营养细胞和假定产肠毒素阴性菌株产生分子量为34 kDa的肠毒素的能力的报道提出了质疑。
