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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Donnelly L E, Cryer A, Butterwith S C

Department of Biochemistry, College of Cardiff, University of Wales.

Br Poult Sci. 1993 Mar;34(1):187-93. doi: 10.1080/00071669308417574.

Putative adipocyte precursor cells were isolated from the white adipose tissue of young broiler and layer chickens and cultured in vitro. 2. The cells from both sources were shown to have the characteristics of adipocyte precursor cells. On reaching confluence, lipoprotein lipase activity was induced and the cells from both strains accumulated large amounts of lipid in the presence of chicken serum. 3. Measurement of cell number over time in culture and calculation of cell doubling times showed that cells from broilers proliferated at a faster rate than those derived from layer-strain chickens. This was the case whether primary or secondary cell cultures were used. Primary cultures of broiler cells had a doubling time of 22 h versus 39 h for layer cells. 4. The contribution of such a difference in proliferative rate to the differential rate of adipose tissue growth between broiler and layer strains observed in vivo is discussed.

从幼年肉鸡和蛋鸡的白色脂肪组织中分离出假定的脂肪细胞前体细胞，并进行体外培养。2. 来自这两种来源的细胞均显示出脂肪细胞前体细胞的特征。达到汇合状态时，诱导脂蛋白脂肪酶活性，并且在鸡血清存在下，这两种品系的细胞都积累了大量脂质。3. 对培养过程中细胞数量随时间的测量以及细胞倍增时间的计算表明，肉鸡来源的细胞比蛋鸡品系来源的细胞增殖速度更快。无论是原代细胞培养还是传代细胞培养，都是如此。肉鸡细胞原代培养的倍增时间为22小时，而蛋鸡细胞为39小时。4. 讨论了这种增殖速率差异对体内观察到的肉鸡和蛋鸡品系之间脂肪组织生长差异速率的贡献。

Donnelly L E, Cryer A, Butterwith S C

Department of Biochemistry, College of Cardiff, University of Wales.

Br Poult Sci. 1993 Mar;34(1):187-93. doi: 10.1080/00071669308417574.

Putative adipocyte precursor cells were isolated from the white adipose tissue of young broiler and layer chickens and cultured in vitro. 2. The cells from both sources were shown to have the characteristics of adipocyte precursor cells. On reaching confluence, lipoprotein lipase activity was induced and the cells from both strains accumulated large amounts of lipid in the presence of chicken serum. 3. Measurement of cell number over time in culture and calculation of cell doubling times showed that cells from broilers proliferated at a faster rate than those derived from layer-strain chickens. This was the case whether primary or secondary cell cultures were used. Primary cultures of broiler cells had a doubling time of 22 h versus 39 h for layer cells. 4. The contribution of such a difference in proliferative rate to the differential rate of adipose tissue growth between broiler and layer strains observed in vivo is discussed.

从幼年肉鸡和蛋鸡的白色脂肪组织中分离出假定的脂肪细胞前体细胞，并进行体外培养。2. 来自这两种来源的细胞均显示出脂肪细胞前体细胞的特征。达到汇合状态时，诱导脂蛋白脂肪酶活性，并且在鸡血清存在下，这两种品系的细胞都积累了大量脂质。3. 对培养过程中细胞数量随时间的测量以及细胞倍增时间的计算表明，肉鸡来源的细胞比蛋鸡品系来源的细胞增殖速度更快。无论是原代细胞培养还是传代细胞培养，都是如此。肉鸡细胞原代培养的倍增时间为22小时，而蛋鸡细胞为39小时。4. 讨论了这种增殖速率差异对体内观察到的肉鸡和蛋鸡品系之间脂肪组织生长差异速率的贡献。