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一些狐猴科动物中高度重复DNA序列的比较及其分类学意义

Comparison of highly repeated DNA sequences in some Lemuridae and taxonomic implications.

作者信息

Montagnon D, Crovella S, Rumpler Y

机构信息

Institut d'Embryologie, Université Louis Pasteur, Faculté de Médecine, Strasbourg, France.

出版信息

Cytogenet Cell Genet. 1993;63(2):131-4. doi: 10.1159/000133518.

Abstract

Highly repeated DNA sequences of Eulemur fulvus mayottensis, E. coronatus, Lemur catta, and Hapalemur griseus griseus have been identified and compared. Sequence analysis of highly repeated DNA fragments isolated from L. catta and Hapalemur showed a high percentage of similarity (nearly 95%), as did fragments isolated from the two very close Eulemur species, whereas comparison of the DNA fragments isolated from the two Eulemur species and the L. catta/Hapalemur group showed a very low percentage (approximately 40%) of identity, as might be expected for distant species. These results confirm our previous data, obtained by Southern blot hybridization techniques on the same species, and strongly support the existence of a common trunk between L. catta and Hapalemur, but different from the leading to the Eulemur species.

摘要

已对马约特狐猴、冠狐猴、环尾狐猴和灰鼠狐猴的高度重复DNA序列进行了鉴定和比较。从环尾狐猴和灰鼠狐猴中分离出的高度重复DNA片段的序列分析显示出很高的相似百分比(近95%),从两个亲缘关系非常近的狐猴物种中分离出的片段也是如此,而从这两个狐猴物种与环尾狐猴/灰鼠狐猴组中分离出的DNA片段的比较显示同一性百分比非常低(约40%),这对于远缘物种来说是可以预料的。这些结果证实了我们之前通过对同一物种进行Southern印迹杂交技术获得的数据,并有力地支持了环尾狐猴和灰鼠狐猴之间存在一个共同主干,但与通向狐猴物种的主干不同。

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