胆碱转运体是分离的大鼠肝线粒体中胆碱氧化控制的主要位点。
The choline transporter is the major site of control of choline oxidation in isolated rat liver mitochondria.
作者信息
Kaplan C P, Porter R K, Brand M D
机构信息
Department of Biochemistry, University of Cambridge, UK.
出版信息
FEBS Lett. 1993 Apr 19;321(1):24-6. doi: 10.1016/0014-5793(93)80613-y.
The degree of control exerted by the mitochondrial choline transporter over the oxidation pathway was measured in isolated rat liver mitochondria. Choline transporter activity was titrated with hemicholinium-3, a known competitive inhibitor of the transporter. It was shown that the rate of betaine efflux from mitochondria was an accurate measure of choline oxidation. The relative rate of choline oxidation was measured as a function of the relative degree of inhibition of the transporter. The resulting data gave a flux control coefficient over choline oxidation of 0.9 for the choline transporter. It is concluded that the choline transporter is the major site for control of choline oxidation in isolated rat liver mitochondria.
在分离的大鼠肝脏线粒体中测量了线粒体胆碱转运体对氧化途径的控制程度。用半胱氨酸-3（一种已知的转运体竞争性抑制剂）滴定胆碱转运体活性。结果表明，线粒体中甜菜碱的流出速率是胆碱氧化的准确指标。胆碱氧化的相对速率作为转运体相对抑制程度的函数进行测量。所得数据表明，胆碱转运体对胆碱氧化的通量控制系数为0.9。结论是，胆碱转运体是分离的大鼠肝脏线粒体中胆碱氧化控制的主要位点。
Zotero 插件