Hazen K C, Hazen B W
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908.
FEMS Microbiol Lett. 1993 Feb 15;107(1):83-7. doi: 10.1111/j.1574-6968.1993.tb06008.x.
Cell surface hydrophobicity influences pathogenesis of Candida albicans. Previous studies suggested that stationary-phase hydrophilic and hydrophobic cells, obtained by growth at 37 and 23 degrees C, respectively, may have similar hydrophobic proteins. However, whether hydrophilic and hydrophobic surface proteins differ during the growth cycle at 37 degrees C is unknown. Freeze-fracture analysis revealed surface fibrillar layer differences between hydrophobic late-lag and hydrophilic stationary-phase yeast cells grown at 37 degrees C. Hydrophilic protein differences were also observed between these populations. However, similar hydrophobic proteins were detected among the late-lag and stationary phase cells grown at 37 degrees C and hydrophobic stationary-phase cells grown at 23 degrees C. These results suggest that hydrophobic proteins remain constant but hydrophilic proteins vary during growth. Thus, conversion from surface hydrophilicity to hydrophobicity by C. albicans may only require alterations in the hydrophilic fibrillar protein components.
细胞表面疏水性影响白色念珠菌的致病性。先前的研究表明，分别在37℃和23℃生长获得的稳定期亲水性和疏水性细胞可能具有相似的疏水蛋白。然而，在37℃生长周期中亲水性和疏水性表面蛋白是否存在差异尚不清楚。冷冻断裂分析显示，在37℃生长的疏水性延迟后期和亲水性稳定期酵母细胞之间存在表面纤维层差异。在这些群体之间也观察到亲水性蛋白质差异。然而，在37℃生长的延迟后期和稳定期细胞以及在23℃生长的疏水性稳定期细胞中检测到相似的疏水蛋白。这些结果表明，疏水蛋白保持不变，但亲水性蛋白在生长过程中会发生变化。因此，白色念珠菌从表面亲水性向疏水性的转变可能只需要亲水性纤维状蛋白质成分的改变。
