Wetzels A M, Janssen H J, Goverde H J, Bastiaans B A, Takahashi K, Rolland R
Department of Obstetrics and Gynaecology, Catholic University, St Radboud Hospital, Nijmegen, The Netherlands.
Int J Androl. 1993 Feb;16(1):15-9. doi: 10.1111/j.1365-2605.1993.tb01147.x.
To study the effects of sperm density on the results of computer-assisted semen analysis (CASA), 10 washed semen samples were diluted and measured with the CellTrak/S CASA system in a concentration range of 10-180 x 10(6) spermatozoa/ml. All sperm motility parameters were influenced to some extent by sperm density. The motility percentage was influenced significantly in 5 samples (P < 0.005), the straight line velocity in all samples (P < 0.0005 in 7 samples), the curvilinear velocity in 3 samples (P < 0.005), the linearity in 9 samples (P < 0.0005 in 6 samples) and the lateral head displacement in 9 samples (P < 0.005 in 6 samples). In general, the CellTrak/S data are influenced significantly if sperm density exceeds 50 x 10(6) spermatozoa/ml. The influence of sperm density on the motility parameters can be explained both by the accuracy of the CASA system and by actual changes in the motility of the spermatozoa. In the light of other published studies, it is concluded that sperm motility measurements with CASA systems should be assessed using 25-50 x 10(6) spermatozoa/ml, especially in studies concerning lateral head displacement and the linearity, as in sperm hyperactivation studies.
为研究精子密度对计算机辅助精液分析（CASA）结果的影响，对10份洗涤后的精液样本进行稀释，并使用CellTrak/S CASA系统在10 - 180×10⁶精子/ml的浓度范围内进行测量。所有精子活力参数在一定程度上均受精子密度影响。5份样本的活力百分比受到显著影响（P < 0.005），所有样本的直线速度受到影响（7份样本中P < 0.0005），3份样本的曲线速度受到影响（P < 0.005），9份样本的直线性受到影响（6份样本中P < 0.0005），9份样本的头部侧向位移受到影响（6份样本中P < 0.005）。一般而言，如果精子密度超过50×10⁶精子/ml，CellTrak/S数据会受到显著影响。精子密度对活力参数的影响既可以通过CASA系统的准确性来解释，也可以通过精子活力的实际变化来解释。根据其他已发表的研究得出结论，使用CASA系统测量精子活力时，应采用25 - 50×10⁶精子/ml进行评估，尤其是在涉及头部侧向位移和直线性的研究中，如精子超激活研究。
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