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干酪乳杆菌中编码缬氨酰 - tRNA合成酶的valS基因的克隆与序列测定。

Cloning and sequence determination of the valS gene, encoding valyl-tRNA synthetase in Lactobacillus casei.

作者信息

Taylor B V, Toy J, Sit T L, Bognar A L

机构信息

Department of Microbiology, University of Toronto, Ontario, Canada.

出版信息

J Bacteriol. 1993 Apr;175(8):2475-8. doi: 10.1128/jb.175.8.2475-2478.1993.

DOI:10.1128/jb.175.8.2475-2478.1993
PMID:8468307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204542/
Abstract

The DNA sequence of the valS gene from Lactobacillus casei and the predicted amino acid sequence of its valyl-tRNA synthetase product have been determined. An open reading frame coding for a protein of 901 amino acids was found. A clone containing the intact L. casei valS gene functionally complemented the temperature-sensitive growth of the valS mutant strain 236c of Escherichia coli. The valS gene and the downstream folylpolyglutamate synthetase gene are transcribed in the same direction but are separated by a putative transcription terminator.

摘要

已确定干酪乳杆菌valS基因的DNA序列及其缬氨酰-tRNA合成酶产物的预测氨基酸序列。发现了一个编码901个氨基酸的蛋白质的开放阅读框。含有完整干酪乳杆菌valS基因的克隆在功能上补充了大肠杆菌valS突变株236c的温度敏感生长。valS基因和下游的叶酰聚谷氨酸合成酶基因同向转录，但被一个推定的转录终止子隔开。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffab/204542/663ecade119a/jbacter00050-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffab/204542/663ecade119a/jbacter00050-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffab/204542/663ecade119a/jbacter00050-0318-a.jpg

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J Bacteriol. 1997 Apr;179(8):2472-8. doi: 10.1128/jb.179.8.2472-2478.1997.

本文引用的文献

1
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
2
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
3
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
4
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
5
Identification of the uvrA gene product.uvrA基因产物的鉴定。
J Mol Biol. 1981 May 5;148(1):45-62. doi: 10.1016/0022-2836(81)90234-5.
6
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
7
Molecular cloning of the gene encoding the valyl-tRNA synthetase from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌缬氨酰 - tRNA合成酶编码基因的分子克隆
Gene. 1986;44(1):139-42. doi: 10.1016/0378-1119(86)90053-3.
8
Crystal structure of a deletion mutant of a tyrosyl-tRNA synthetase complexed with tyrosine.与酪氨酸复合的酪氨酰-tRNA合成酶缺失突变体的晶体结构。
J Mol Biol. 1987 Mar 20;194(2):287-97. doi: 10.1016/0022-2836(87)90376-7.
9
The valyl-tRNA synthetase from Bacillus stearothermophilus has considerable sequence homology with the isoleucyl-tRNA synthetase from Escherichia coli.嗜热脂肪芽孢杆菌的缬氨酰 - tRNA合成酶与大肠杆菌的异亮氨酰 - tRNA合成酶具有相当程度的序列同源性。
Biochemistry. 1987 May 5;26(9):2480-6. doi: 10.1021/bi00383a012.
10
Structure of the yeast valyl-tRNA synthetase gene (VASI) and the homology of its translated amino acid sequence with Escherichia coli isoleucyl-tRNA synthetase.酵母缬氨酰 - tRNA合成酶基因（VASI）的结构及其翻译的氨基酸序列与大肠杆菌异亮氨酰 - tRNA合成酶的同源性。
J Biol Chem. 1987 May 25;262(15):7189-94.