嗜热脂肪芽孢杆菌缬氨酰 - tRNA合成酶编码基因的分子克隆
Molecular cloning of the gene encoding the valyl-tRNA synthetase from Bacillus stearothermophilus.
作者信息
Brand N J, Fersht A R
出版信息
Gene. 1986;44(1):139-42. doi: 10.1016/0378-1119(86)90053-3.
PMID:3770480
Abstract
The valS gene from the thermophile Bacillus stearothermophilus encoding the valyl-tRNA synthetase has been cloned on a 13.8-kb plasmid. The gene product and its kinetic properties are comparable with those of the native enzyme.
摘要
编码缬氨酰 - tRNA合成酶的嗜热脂肪芽孢杆菌的valS基因已被克隆到一个13.8 kb的质粒上。该基因产物及其动力学特性与天然酶相当。
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