采用固相萃取和高效液相色谱法以及高效液相色谱-粒子束质谱法测定血清中的尼古丁及其两种主要代谢物。
Determination of nicotine and two major metabolites in serum by solid-phase extraction and high-performance liquid chromatography, and high-performance liquid chromatography-particle beam mass spectrometry.
作者信息
Pacifici R, Pichini S, Altieri I, Rosa M, Bacosi A, Caronna A, Zuccaro P
机构信息
Ospedale Santo Spirito, Rome, Italy.
出版信息
J Chromatogr. 1993 Feb 26;612(2):209-13. doi: 10.1016/0378-4347(93)80165-z.
A rapid and selective assay of nicotine, cotinine and trans-3'-hydroxycotinine in human serum, based on high-performance liquid chromatography with UV detection has been developed. The compounds were subjected to solid-phase extraction, using Extrelut 1 cartridges. Recoveries were ca. 95% for nicotine, 90% for cotinine and 50-55% for trans-3'-hydroxycotinine. The limit of quantitation observed with this method was 10 ng/ml for nicotine and 5 ng/ml for each of the metabolites. The compounds were also identified using high-performance liquid chromatography with particle beam mass spectrometry, to confirm their presence in human serum.
基于高效液相色谱-紫外检测法，开发了一种快速、选择性地检测人血清中尼古丁、可替宁和反式-3'-羟基可替宁的方法。使用Extrelut 1柱对这些化合物进行固相萃取。尼古丁的回收率约为95%，可替宁为90%，反式-3'-羟基可替宁为50 - 55%。该方法的定量限为尼古丁10 ng/ml，每种代谢物5 ng/ml。还使用高效液相色谱-粒子束质谱法对这些化合物进行了鉴定，以确认它们在人血清中的存在。
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