Zuccaro P, Altieri I, Rosa M, Passa A R, Pichini S, Ricciarello G, Pacifici R
Istituto Superiore di Sanità, Rome, Italy.
J Chromatogr. 1993 Nov 24;621(2):257-61. doi: 10.1016/0378-4347(93)80103-b.
A simple and reliable reversed-phase high-performance liquid chromatographic method with ultraviolet detection is described for the quantitation of nicotine and its metabolites cotinine, trans-3'-hydroxycotinine, norcotinine and cotinine N-oxide in human serum. The analytes and the internal standard, N-ethylnorcotinine, were extracted by solid-phase extraction before chromatography. Two different columns and mobile phases with gradient systems were used. The detection limit of the assay was 10 ng/ml for nicotine, 3 ng/ml for cotinine N-oxide and 5 ng/ml for cotinine, trans-3'-hydroxycotinine and norcotinine. The concentrations of nicotine and its metabolites in the serum of 12 cigarette smokers are reported.
描述了一种简单可靠的反相高效液相色谱法，采用紫外检测，用于定量测定人血清中的尼古丁及其代谢物可替宁、反式-3'-羟基可替宁、去甲可替宁和可替宁N-氧化物。分析物和内标N-乙基去甲可替宁在色谱分析前通过固相萃取进行提取。使用了两种不同的色谱柱和带有梯度系统的流动相。该测定法的检测限为尼古丁10 ng/ml、可替宁N-氧化物3 ng/ml、可替宁、反式-3'-羟基可替宁和去甲可替宁5 ng/ml。报告了12名吸烟者血清中尼古丁及其代谢物的浓度。
