Titulaer H A, Vink-Blijleven N
Department of Pharmaceutics, Faculty of Pharmacy, Utrecht University, Netherlands.
J Chromatogr. 1993 Feb 26;612(2):331-5. doi: 10.1016/0378-4347(93)80182-4.
A simple and rapid high-performance liquid chromatographic method with UV detection for the determination in serum of the antimalarial artelinic acid is described. Artelinic acid is a water-soluble semi-synthetic derivative of artemisinin (or Qinghaosu), which is the parent compound of a completely new class of antimalarials with great potential value. Calibration curves of artelinic acid in serum from 0.5 to 50 micrograms/ml were found to be linear, and the extraction recovery was ca. 70% from rabbit serum and 45% from bovine and human serum. The detection limit of the method was 50 ng/ml when 250 microliters of serum were processed. Spiked samples (rabbit serum) proved to be stable for at least 43 days on storage at -20 degrees C. The method has been used for the analysis of ca. 400 samples in a pharmacokinetic study. About 50 samples can be processed in half a day.
描述了一种简单快速的高效液相色谱法，采用紫外检测，用于测定血清中的抗疟药物青蒿琥酯。青蒿琥酯是青蒿素（或青蒿素）的水溶性半合成衍生物，青蒿素是一类具有巨大潜在价值的全新抗疟药物的母体化合物。血清中青蒿琥酯浓度在0.5至50微克/毫升之间的校准曲线呈线性，从兔血清中的提取回收率约为70%，从牛血清和人血清中的提取回收率为45%。当处理250微升血清时，该方法的检测限为50纳克/毫升。加标样品（兔血清）在-20℃储存时至少43天稳定。该方法已用于一项药代动力学研究中约400个样品的分析。半天左右可以处理约50个样品。
