Sar M, Lubahn D B, French F S, Wilson E M
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
Endocrinology. 1990 Dec;127(6):3180-6. doi: 10.1210/endo-127-6-3180.
Immunohistochemical localization of the androgen receptor (AR) was performed in reproductive tissues, submaxillary gland, pituitary, and brain of the rat and in human prostate. AR was visualized using either of two polyclonal antibodies raised against peptides with sequences derived from rat and human AR. Tissue sections of 6-8 microns, frozen in isopentane and fixed in paraformaldehyde, were stained using immunoglobulin G fractions of immune, preimmune, and peptide-adsorbed immune sera in the avidin-biotin peroxidase procedure. AR was prominent in nuclei of acinar epithelial cells of epididymis, ventral prostate, seminal vesicle, and ductus deferens from the intact rat. Androgen withdrawal, 3 days after castration, resulted in the loss of receptor immunostaining, which was restored within 15 min of androgen administration. Stromal cell staining was absent or weak in the ventral prostate of intact rats, but was more evident in the epididymis. AR was confined to nuclei of cells within and bordering the interstitial compartment of the testis, including Sertoli cells, peritubular myoid cells, and interstitial cells, and was undetectable in germ cells. Submaxillary gland epithelial cells and a population of rat anterior pituitary cells showed strong nuclear staining of AR. In rat brain, AR was present in the medial preoptic, arcurate, and ventromedial nuclei of the hypothalamus, the medial nucleus of the amygdala, the CA-1 hippocampus, and the cortex. AR was prominent in acinar epithelial cells in human benign prostatic hyperplasia and was also present in stroma of fibromuscular benign hyperplasia. Heterogeneous staining was observed in stromal and epithelial cells of prostatic adenocarcinoma. The results of these studies indicate that AR can be detected immunohistochemically in a variety of tissues and cell types using antipeptide polyclonal antibodies. The presence of AR in tissues correlated with their known androgen responsiveness.
在大鼠的生殖组织、颌下腺、垂体、脑以及人类前列腺中进行了雄激素受体（AR）的免疫组织化学定位。使用针对源自大鼠和人类AR序列的肽段制备的两种多克隆抗体之一来观察AR。将6 - 8微米厚的组织切片在异戊烷中冷冻并在多聚甲醛中固定，采用抗生物素蛋白 - 生物素过氧化物酶法，用免疫血清、免疫前血清和肽吸附免疫血清的免疫球蛋白G组分进行染色。在完整大鼠的附睾、腹侧前列腺、精囊和输精管的腺泡上皮细胞核中，AR很显著。去势3天后雄激素撤离导致受体免疫染色消失，而在给予雄激素后15分钟内恢复。在完整大鼠的腹侧前列腺中，基质细胞染色缺失或较弱，但在附睾中更明显。AR局限于睾丸间质隔内及周边细胞的细胞核，包括支持细胞、睾丸肌样细胞和间质细胞，在生殖细胞中未检测到。颌下腺上皮细胞和一群大鼠垂体前叶细胞显示出强烈的AR核染色。在大鼠脑中，AR存在于下丘脑的内侧视前核、弓状核和腹内侧核、杏仁核内侧核、海马CA - 1区和皮质。在人类良性前列腺增生的腺泡上皮细胞中AR很显著，在纤维肌肉良性增生的基质中也存在。在前列腺腺癌的基质和上皮细胞中观察到异质性染色。这些研究结果表明，使用抗肽多克隆抗体可通过免疫组织化学在多种组织和细胞类型中检测到AR。组织中AR的存在与其已知的雄激素反应性相关。
