从全长三叶草黄花叶病毒cDNA克隆体产生传染性体外转录本。

Production of infectious in vitro transcripts from a full-length clover yellow mosaic virus cDNA clone.

作者信息

Holy S, AbouHaidar M G

机构信息

Department of Botany, University of Toronto, Ontario, Canada.

出版信息

J Gen Virol. 1993 Apr;74 ( Pt 4):781-4. doi: 10.1099/0022-1317-74-4-781.

DOI:10.1099/0022-1317-74-4-781

PMID:8468561

Abstract

A full-length cDNA copy of clover yellow mosaic virus (CYMV) RNA was constructed from two smaller cDNA clones. In vitro transcription of linearized plasmid with T7 RNA polymerase produced genomic-sized RNA. These transcripts caused symptoms typical of CYMV infection when used to inoculate both a systemic host (Vicia faba) and a local lesion host (Gomphrena globosa). Electron microscopy of extracts from individual local lesions revealed virus particles identical to native CYMV. Increasing the length of the poly(A) tail from 23 residues to 80 or to 135 residues increased the infectivity rate from 12% to 17% or to 35% that of native CYMV RNA, respectively.

摘要

从两个较小的 cDNA 克隆构建了三叶草黄花叶病毒（CYMV）RNA 的全长 cDNA 拷贝。用 T7 RNA 聚合酶对线性化质粒进行体外转录产生了基因组大小的 RNA。当用于接种系统宿主（蚕豆）和局部病斑宿主（千日红）时，这些转录本引发了典型的 CYMV 感染症状。对单个局部病斑提取物的电子显微镜观察显示出与天然 CYMV 相同的病毒颗粒。将 poly(A) 尾的长度从 23 个残基增加到 80 个或 135 个残基，感染率分别从天然 CYMV RNA 的 12% 提高到 17% 或 35%。