Changes with ageing in total dolichol and dolichol fractions in Drosophila.

Since it is unclear how the concentration of dolichol fractions change with ageing in mammals, we have examined the changes in another organism, Drosophila. Dolichol extracted from Drosophila melanogaster was found to consist of three fractions composed of 15, 16 and 17 isoprene units. The total dolichol content of female flies maintained at 25 degrees C increased with ageing between 0 and 64 days of adult age but the change was not significant. The total dolichol content of male flies decreased with ageing but the decrease was not significant. The relative amounts of the three different dolichol fractions in both male and female flies also failed to show any significant ageing-related change. The greatest amount of dolichol was found in the 16 isoprene unit fraction representing 67.2% of the total dolichol in male flies and 65.4% in female flies. Increased dietary dolichol had little or no influence on the life span of Drosophila when given either during the developmental or adult stages.