Marranzano M, Agodi A, Gulisano M
Institute of Hygiene and Preventive Medicine, University of Catania, Italy.
New Microbiol. 1993 Jan;16(1):57-62.
Thirty-seven strains of Yersinia isolated from human and environmental sources were investigated for virulence and molecular characteristics. Clinical isolates of Y. enterocolitica serotypes 0:3 and 0:9, harbouring the 45 MDa virulence plasmid were Ca++ dependent and responded positively to the CV-binding assay. The environmental Y. kristensenii strain, although harbouring a 45 MDa plasmid proved to be negative for both assays. All the environmental strains showed pyrazinamidase activity, while isolates from human sources proved negative. Two different plasmid profiles were disclosed: one Y. enterocolitica strain (serotype 0:7, 8) showed one plasmid of 67 MDa and one plasmid of 1.5 MDa. Eleven human isolates of Y. enterocolitica and one environmental strain of Y. krinstensenii showed only one plasmid of 45 MDa. Restriction endonuclease analysis of the 45 MDa plasmids supported the hypothesis that the environmental Y. krinstensenii plasmid is unrelated to the family of homologous virulence plasmids of Y. enterocolitica.
对从人类和环境来源分离出的37株耶尔森氏菌进行了毒力和分子特征研究。携带45 MDa毒力质粒的小肠结肠炎耶尔森氏菌血清型0:3和0:9临床分离株对钙离子有依赖性，并且对CV结合试验呈阳性反应。环境来源的克氏耶尔森氏菌菌株，尽管携带一个45 MDa质粒，但两种试验均呈阴性。所有环境菌株均显示吡嗪酰胺酶活性，而来自人类来源的分离株呈阴性。揭示了两种不同的质粒图谱：一株小肠结肠炎耶尔森氏菌菌株（血清型0:7, 8）显示一个67 MDa的质粒和一个1.5 MDa的质粒。11株小肠结肠炎耶尔森氏菌人类分离株和一株克氏耶尔森氏菌环境菌株仅显示一个45 MDa的质粒。对45 MDa质粒的限制性内切酶分析支持了以下假设：环境来源的克氏耶尔森氏菌质粒与小肠结肠炎耶尔森氏菌同源毒力质粒家族无关。
