Horiki T, Moriuchi J, Inoko H, Morita K, Tsuji K, Shinohara Y, Ichikawa Y, Arimori S
Department of Internal Medicine IV, School of Medicine, Tokai University, Kanagawa, Japan.
Neurology. 1993 Apr;43(4):771-4. doi: 10.1212/wnl.43.4.771.
We investigated the HLA-DPB1 allele in 43 unrelated Japanese patients with myasthenia gravis (MG) using digestion of polymerase chain reaction (PCR)-amplified DNA with allele-specific restriction endonucleases (PCR-RFLP method). We found a higher frequency of the DPB10201 allele in female patients whose ages at onset were less than 30 years (83.3%) than in controls (35.6%). The study also included serologic typing of HLA-A, -B, -C, and -DR antigens in 72 patients with MG, and confirmed previous results demonstrating a strong association of HLA-DR53 with early onset of MG in females. These results indicate that both the DPB10201 allele and DR53 play key roles in the disease process of MG in early-onset females, and that the genetic background of Japanese females with early-onset MG is different from that of other patients with MG.
我们采用等位基因特异性限制性内切酶消化聚合酶链反应（PCR）扩增的DNA的方法（PCR-RFLP法），对43例无亲缘关系的日本重症肌无力（MG）患者的HLA-DPB1等位基因进行了研究。我们发现，发病年龄小于30岁的女性患者中DPB10201等位基因的频率（83.3%）高于对照组（35.6%）。该研究还对72例MG患者进行了HLA-A、-B、-C和-DR抗原的血清学分型，并证实了先前的结果，即HLA-DR53与女性MG的早发密切相关。这些结果表明，DPB10201等位基因和DR53在早发女性MG的疾病过程中均起关键作用，且早发MG的日本女性的遗传背景与其他MG患者不同。
